Dr. Christopher Stanley

Dr. Christopher Stanley

Clifford Shull Fellow

Instrument Scientist: EQ-SANS

Education

1999–2004 Ph.D. Polymer Science and Engineering, University of Massachusetts, Amherst, MA
1995–1999 B.S. Polymer and Textile Chemistry, Georgia Institute of Technology, Atlanta, GA

B. Positions and Honors Positions

2011–present Instrument Scientist, EQ-SANS, Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, TN

2011–present Joint Faculty Assistant Professor, Dept. of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, TN

2007–2011 Clifford G. Shull Research Fellow, Neutron Scattering Science Division, Oak Ridge National Laboratory, Oak Ridge, TN

2004–2007 National Research Council Postdoctoral Research Associate, NIST Center for Neutron Research, Gaithersburg, MD. Advisor: Susan Krueger
Joint with the Laboratory of Physical and Structural Biology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD. Collaborators: V. Adrian Parsegian and Donald C. Rau (NIH)

1999–2004 Graduate Research Assistant, Dept. of Polymer Science and Engineering, University of Massachusetts, Amherst, MA. Thesis Title: Interactions of Chiral Biopolymers.
Advisor: Helmut H. Strey

1998–1999 Undergraduate Research Assistant, Dept. of Textile and Fiber Engineering, Georgia Institute of Technology, Atlanta, GA. Advisor: Haskell W. Beckham

Awards and Honors

2007–present Clifford G. Shull Research Fellowship, Neutron Scattering Science Division, Oak Ridge National Laboratory, Oak Ridge, TN.

2004–2007 National Research Council Postdoctoral Research Associateship, NIST Center for Neutron Research, Gaithersburg, MD.

2000 Santos Go Memorial Scholarship, Polymer Science and Engineering Department, University of Massachusetts, Amherst, MA.

1995–1999 HOPE Scholarship, Georgia Institute of Technology, Atlanta, GA

Professional Activities

Reviewer:

Agence Nationale de la Recherche grant proposal, France (2011)

SANS and USANS beam time proposals for NIST Center for Neutron Research, Gaithersburg, MD, (2007–present)

Advisory Member, Soft Condensed Matter Committee Group, NIST Center for Neutron Research Expansion Workshop, Bethesda, MD (2006)

Biophysical Journal
Journal of Applied Crystallography

Mentoring:

Ph.D. thesis committee: Tatiana Perevozchikova, Graduate School of Medicine, University of Tennessee Medical Center, Knoxville, TN (2008–2012)

Postdoctoral trainees: Erica Rowe (2009–2011)

Undergraduate students: Laura Grese (6/2009–7/2010), Zac Enderson (summer 2010), Amanda DeBuhr (1/2011-5/2012)

Memberships:

American Chemical Society, American Physical Society, Biophysical Society, Neutron Scattering Society of America

Selected Talks and Workshops

National:

  • Small-angle neutron scattering to investigate huntingtin protein aggregation in Huntington’s disease, Meeting of the American Crystallographic Association, Session: Fibril-forming pathological peptides; Boston, MA; Jul. 29, 2012.

  • Small-angle neutron scattering on the EQ-SANS instrument at the SNS, American Conference on Neutron Scattering, Session: Sources, Instrumentation and Software; Washington, DC; Jun. 25, 2012.

  • Structural flexibility of intrinsically disordered proteins, IUPUI Workshop on Biological Physics, Physics Department, Indiana University Purdue University Indianapolis, Indianapolis, IN; Aug. 16, 2010.

  • Neutrons, Huntington's disease, and life in Oak Ridge, Tennessee, Physics in Biology - Mini Symposium in Honor of V. Adrian Parsegian, National Institutes of Health, Bethesda, MD; May 28, 2009.

  • Unraveling the polyglutamine aggregation pathway in Huntington’s disease by small-angle neutron scattering, Biophysical Society 53th Annual Meeting, Session: Amyloids from Multiple Perspectives; Boston, MA; Mar. 2, 2009.

  • Small-angle neutron scattering to investigate protein aggregation in Huntington's disease, Physics Diversity Summit and the 2009 Joint Annual Conference of the National Society of Black Physicists and the National Society of Hispanic Physicists, Nashville, TN; Feb. 13, 2009.

  • Polyglutamine aggregation studied by small-angle neutron scattering, 22nd Symposium of the Protein Society, Session: Amyloid Structure and Formation: From Test Tubes to Cells and Tissues; San Diego, CA; July 19, 2008.

Local:

  • The utility of SANS to investigate challenging protein systems: from disorder to aggregation to hydration, Graduate Course on Neutrons in Biology, ORNL, Oak Ridge, TN; Jun. 4, 2012.

  • Characterizing intrinsically disordered proteins by small-angle neutron scattering, Graduate Course on Intrinsically Disordered Proteins, Biochemistry and Cellular and Molecular Biology Department, University of Tennessee, Knoxville, TN; Apr. 27, 2011.

  • SANS to investigate protein aggregation, disorder, and hydration, Seminar in the Biochemistry and Cellular and Molecular Biology Department, University of Tennessee, Knoxville, TN; Mar. 10, 2011.

  • Small-angle neutron scattering to investigate the polyglutamine aggregation pathway in Huntington's disease, Graduate Course on Neutrons in Biology, ORNL, Oak Ridge, TN; Jun. 7, 2010.

  • Utilizing small-angle neutron scattering to investigate the polyglutamine aggregation pathway in Huntington's disease, 7th Annual SER-CAT Meeting, ORNL, Oak Ridge, TN; Mar. 19, 2010.

  • Protein hydration and association studied by small-angle neutron scattering, Comparative and Experimental Medicine and Public Health Research Symposium, University of Tennessee, Knoxville, TN; Jun. 15, 2009.

C. Book Chapters

Todd, B. A.; Sidorova, N. Y.; Stanley, C. B.; Rau, D. C. Hydration Forces, Chapter for Wiley Encyclopedia of Chemical Biology, John Wiley and Sons, Inc., 2008.

D. Peer-Reviewed Publications

  • Burger, V. M.; Ramanathan, A.; Savol, A. J.; Stanley, C. B.; Agarwal, P. K.; Chennubhotla, C. S. Quasi-anharmonic analysis reveals intermediate states in the nuclear co-activator receptor binding domain ensemble. Pac. Symp. Biocomput. 2012, 17, 70–81.

  • Stanley, C. B.; Rau, D. C. Evidence for water restructuring forces between surfaces. Invited review article: Curr. Opin. Colloid Interface Sci. 2011, 16, 551–556.

  • Stanley, C. B.; Perevozchikova, T.; Berthelier, V. Structural formation of huntingtin exon 1 aggregates probed by small-angle neutron scattering. Biophys. J. 2011, 100, 2504–2512.

  • Rubinson, K.; Stanley, C.; Krueger, S. Small-angle neutron scattering and the errors in protein structures that arise from uncorrected background and intermolecular interactions. J. Appl. Cryst., 2008, 41, 456–465.

  • Leisen, J.; Schauss, G.; Stanley, C.; Beckham, H. W. Magnetic resonance imaging applications in textile and fiber engineering: fabrics and diapers, AATCC Rev., 2008, 8, 32–37.

  • Stanley, C.; Rau, D. C. Assessing the interaction of urea and protein-stabilizing osmolytes with the nonpolar surface of hydroxypropylcellulose, Biochemistry 2008, 47, 6711–6718.

  • Stanley, C. B.; Strey, H. H. Osmotically induced helix-coil transition in poly(glutamic acid), Biophys. J., 2008, 94, 4427–4434.

  • Stanley, C. B.; Krueger, S.; Parsegian, V. A.; Rau, D. C. Protein structure and hydration probed by SANS and osmotic stress, Biophys. J. 2008, 94, 2777–2789.

  • Stanley, C. B.; Strey, H. H. Electrostatically driven self-assembly of hybrid DNA-elastin liquid crystals, Soft Matter 2008, 4, 241–244.

  • Chaiyasit, W.; Stanley, C. B.; Strey, H. H.; McClements, D. J.; Decker, E. A. Impact of surface active compounds on iron catalyzed oxidation of methyl linolenate in AOT–water–hexadecane systems. Food Biophys. 2007, 2, 57–66.

  • Stanley, C. B.; Rau, D. C. Preferential hydration of DNA: The magnitude and distance dependence of alcohol and polyol interactions. Biophys. J. 2006, 91, 912–920.

  • Stanley, C. B.; Hong, H.; Strey, H. H. DNA cholesteric pitch as a function of density and ionic strength. Biophys. J. 2005, 89, 2552–2557.

  • Stanley, C. B.; Strey, H. H. Measuring osmotic pressure of poly(ethylene glycol) solutions by sedimentation equilibrium ultracentrifugation. Macromolecules 2003, 36, 6888–6893.

  • Sturgill, G. K.; Stanley, C.; Rezac, M. E.; Beckham, H. W. Solid-state complexes of hexafluoro-2- propanol with benzophenone-containing polyimides. Macromolecules 2001, 34, 8730–8734.

  • Pschirer N. G.; Marshall A. R.; Stanley C.; Beckham H. W.; Bunz U. H. F. Synthesis and characterization of poly[1,5-(3,7-di-tert-butyl)naphthyleneethynylene] by alkyne metathesis. Macromol. Rapid Commun. 2000, 21, 493–495.

E. Research Support

Previous Support:

ORNL LDRD-5246
Laboratory Directed Research and Development (LDRD), Neutron Sciences
Role: PI
Funding: $710k (10/1/2008–9/30/2011)
Project Title: Neutron Scattering and Osmotic Stress to Study Intrinsically Disordered Proteins.