Kevin Weiss

Kevin Weiss

Position: Bio-Deuteration Lab Manager and Technical Lead

Group: Energy and the Environment

Facility: Spallation Neutron Source


Phone: (865) 805-7388

2008-present ​Technical Staff, Center for Structural Molecular Biology Oak Ridge National Laboratory 2005-2008 Postdoctoral Research Associate, Center for Structural Molecular Biology Oak Ridge National Laboratory 2001-2005 Postdoctoral Research Associate, Center for Structural Biology Vanderbilt University


Bachelor of Science in Pharmacy, Ohio Northern University, 1992 Ph.D. in Medicinal Chemistry, The Ohio State University, 2001


Protein Expression and Purification Stable Isotope Labeling Fed-Batch Fermentation Neutron Scattering Nuclear Magnetic Resonance Medicinal/OrganicChemistry

Selected Publications

1. Meyn, S.M., Seda, C., Campbell, M., Weiss, K.L., Hu, H., Pastrana-Rios, B. & Chazin, W.J. (2006). The biochemical effect of Ser167 phosphorylation on Chlamydomonas reinhardtii centrin. Biochem Biophys Res Commun, 342 (1), 342-8. 2. Shah, V.N., Wingo, T.L., Weiss, K.L., Williams, C.K., Balser, J.R. & Chazin, W.J. (2006). Calcium-dependent regulation of the voltage-gated sodium channel hH1: intrinsic and extrinsic sensors use a common molecular switch. Proc Natl Acad Sci U S A, 103 (10), 3592-7. 3. Weiss, K.L., Meilleur, F., Blakeley, M.P. & Myles, D.A.A. (2008). Preliminary neutron crystallographic analysis of selectively CH3-protonated deuterated rubredoxin from Pyrococcus furiosus. Acta Crystallogr, Sect F Struct Biol Cryst Commun, 64 (Pt 6), 537-40. 4. Meilleur, F., Weiss, K.L. & Myles, D.A.A. (2009). Deuterium labeling for neutron structure-function-dynamics analysis. Methods Mol Biol, 544, 281-92. 5. Gardberg, A.S., Del Castillo, A R., Weiss, K L., Meilleur, F., Blakeley, M.P. & Myles, D.A.A. (2010). Unambiguous determination of H-atom positions: comparing results from neutron and high-resolution X-ray crystallography. Acta Crystallogr D Biol Crystallogr, 66 (Pt 5), 558-67. 6. Borreguero, J.M., He, J., Meilleur, F., Weiss, K.L., Brown, C M., Myles, D.A.A., Herwig, K.W. & Agarwal, P.K. (2011). Redox-promoting protein motions in rubredoxin. J Phys Chem B, 115 (28), 8925-36. 7. Tomanicek, S.J., Wang, K.K., Weiss, K.L., Blakeley, M.P., Cooper, J., Chen, Y. & Coates, L. (2011). The active site protonation states of perdeuterated Toho-1 beta-lactamase determined by neutron diffraction support a role for Glu166 as the general base in acylation. FEBS Lett, 585 (2), 364-8. 8. Chu, X.Q., Gajapathy, M., Weiss, K.L., Mamontov, E., Ng, J.D. & Coates, L. (2012). Dynamic behavior of oligomeric inorganic pyrophosphatase explored by quasielastic neutron scattering. J Phys Chem B, 116 (33), 9917-21. 9. Clifton, L.A., Johnson, C.L., Solovyova, A.S., Callow, P., Weiss, K.L., Ridley, H., Le Brun, A.P., Kinane, C.J., Webster, J.R., Holt, S.A. & Lakey, J.H. (2012). Low resolution structure and dynamics of a colicin-receptor complex determined by neutron scattering. J Biol Chem, 287 (1), 337-46. 10. Munshi, P., Chung, S.L., Blakeley, M.P., Weiss, K.L., Myles, D.A.A. & Meilleur, F. (2012). Rapid visualization of hydrogen positions in protein neutron crystallographic structures. Acta Crystallogr D Biol Crystallogr, 68 (Pt 1), 35-41. 11. Nickels, J.D., O'Neill, H., Hong, L., Tyagi, M., Ehlers, G., Weiss, K.L., Zhang, Q., Yi, Z., Mamontov, E., Smith, J.C. & Sokolov, A.P. (2012). Dynamics of protein and its hydration water: neutron scattering studies on fully deuterated GFP. Biophys J, 103 (7), 1566-75. 12. Tomanicek, S.J., Standaert, R.F., Weiss, K.L., Ostermann, A., Schrader, T.E., Ng, J.D. & Coates, L. (2013). Neutron and X-ray crystal structures of a perdeuterated enzyme inhibitor complex reveal the catalytic proton network of the Toho-1 beta-lactamase for the acylation reaction. J Biol Chem, 288 (7), 4715-22. 13. Wan, Q., Zhang, Q., Hamilton-Brehm, S., Weiss, K., Mustyakimov, M., Coates, L., Langan, P., Graham, D., Kovalevsky, A., (2013). X-ray crystallographic studies of family 11 xylanase Michaelis and product complexes: implications for the catalytic mechanism. Acta Crystallogr D Biol Crystallogr, 70 (Pt 1),11-23. 14. Wan, Q., Kovalevsky, A., Zhang, Q., Hamilton-Brehm, S., Upton, R., Weiss, K.L., Mustyakimov, M., Graham, D., Coates, L. & Langan, P.A. (2013). Heterologous expression, purification, crystallization and preliminary X-ray analysis of Trichoderma reesei xylanase II and four variants. Acta Crystallogr Sect F Struct Biol Cryst Commun, 69 (Pt 3), 320-3. 15. Coates, L., Tomanicek, S., Schrader, T.E., Weiss, K.L., Ng, J.D., Juttner, P. & Ostermann, A. (2014). Cryogenic neutron protein crystallography: routine methods and potential benefits. J. Appl Crystallogr 47 (4), 1431-1434. 16. Heller, W.T., Urban, V.S., Lynn, G.W., Weiss, K.L., O'Neill, H.M., Pingali, S.V., Qian, S., Littrell, K.C., Melnichenko, Y.B., Buchanan, M.V., Selby, D.L., Wignall, G.D. (2014) The Bio-SANS instrument at the High Flux Isotope Reactor of Oak Ridge National Laboratory. J Appl Crystallogr. 47 (4), 1238-46.

Awards, Honors and Memberships

American Chemical Society